Fig. 6: NDRG1-deficient B cells exposed to self-antigen in the periphery are subject to follicular exclusion and cell death.

a Representative flow cytometry plots of 50:50 mixtures of WT CD45.1⁺ Ig^HEL and WT or Ndrg1^−/− CD45.2⁺ Ig^HEL splenocytes labeled with CTV dye and transferred by i.v. injection into non-tg or sHEL-tg CD45.2⁺ recipients for 48 h, showing live B220⁺CD19⁺ gated populations and % transferred CTV⁺ cells. b Percentage of total lymphocytes that were either CD45.1⁺ or CD45.2⁺ CTV⁺ as gated in (a), with white points showing paired CD45.1⁺ Ig^HEL cells from donor, colored points are CD45.2⁺. c MFI of surface marker expression CD86, CD23, CXCR5, IgM^a, BAFF-R and CD95, and intracellular Bim and Bcl2, in transferred Ig^HEL B cells, gated as in (a). Data points are individual mice and lines show means with 95% CI error bars and data are representative from 1 to 3 independent experiments with 4–6 mice per group. d, e Ratio of B220⁺ cells surviving from 50:50 mixtures of WT CD45.1⁺ and WT or Ndrg1^−/− CD45.2⁺ non-tg B cells stimulated with anti-IgM at the indicated concentrations for 16 h at 37 °C ex vivo (d) and CD86 surface expression as MFI on the CD45.2 + B220⁺ cells (e). Data are representative of 3 independent experiments with columns or circles as means with 95% CI error bars.