Fig. 7: Antigen-experienced T cells respond normally in the absence of NDRG1.

a Experimental schematic; OVA-specific CD4⁺ OT-II⁺ T cells were isolated from CD45.2⁺ WT or Ndrg1^−/− OT-II⁺ tg mice and adoptively transferred to CD45.1⁺ non-tg recipients, with subsequent intravenous injection of 500μg OVA peptide. After 5 days, splenic CD4⁺ cells were isolated from recipient mice and naïve unstimulated WT or Ndrg1^−/− OT-II⁺ tg mice and loaded with CTV. Equal numbers of Vα2⁺ CD45.2⁺OT-II⁺ cells were cultured in vitro with a titration of OVA peptide for 72 h at 37 °C with irradiated APCs. b IL-2 concentration in culture supernatants in (a). c Proliferation of CD4⁺Vα2⁺ CD45.2⁺OT-II⁺ T cells and non-tg bystander WT CD45.1⁺ cells (red) as measured by CTV dilution by flow cytometry. d Quantitation of intracellular IL-2 and IFNγ production by flow cytometry within OT-II⁺ CD4⁺ T cells from (a). Points represent means of technical replicates from one (naïve) or two (antigen-experienced) mice with error bars representing standard deviation. Results are representative of two (b), three (c) or one (d) independent experiment(s).