Fig. 4: Mutational analysis of sensory ATP-binding motifs in NLRP3. | Communications Biology

Fig. 4: Mutational analysis of sensory ATP-binding motifs in NLRP3.

From: ATP-binding and hydrolysis of human NLRP3

Fig. 4: Mutational analysis of sensory ATP-binding motifs in NLRP3.The alternative text for this image may have been generated using AI.

a MBP-NLRP3 wild type and two different Sensor 1 mutants were analyzed by RP-HPLC. The protein samples (3 µM) were incubated at 25 °C in the presence of 100 µM ATP and 5 mM MgCl2. The relative amount of ATP (%) is shown on the y-axis and the time (min) on the x-axis. The amount of ATP was determined in 10 min intervals, integrated and normalized to 100% nucleotide. Sequence alignments and sequence logos of the labeled motifs and residues are shown for human NLRP1-14. b Hydrolysis analysis of MBP-NLRP3 Sensor 2 mutants performed similarly as in a. c ATP hydrolysis analysis of the P412A mutant. d ATP hydrolysis analysis of the R262W CAPS/Glu-switch mutant.

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