Table 2 Correlation of ATP hydrolysis with downstream signaling.

From: ATP-binding and hydrolysis of human NLRP3

NLRP3 protein

ATP hydrolysis

IL-1β secretion

ASC specking

wt

1.00

1.00

1.00

R262W

0.53

0.38

1.78

K232A

0.34

0.08

0.15

T233S

1.21

0.85

1.54

D305A/E306A

0.22

0.16

0.27

R351G

0.40

0.04

0.15

R351T

0.10

0.49

0.13

H522R

0.86

0.07

0.26

  1. Mean values of IL-1β secretion levels of LPS-primed immortalized macrophages and ASC specking (speck/nuclei) determined in HeLa cells transfected with 100 ng of DNA and reconstituted with indicated NLRP3 variants in comparison to ATP turnover numbers of recombinantly expressed NLRP3 variants, all normalized to wild type levels.
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