Fig. 2: Isolation and characterization of EVs from LX-2 cells.

a Size distribution profiles of EVs isolated from differently treated cells (mean ± SD, n = 3). b Quantile subtraction of the yields. c, d SEM (c) and cryo-TEM (d) images of EVs isolated from untreated cells. e–g Protein content and vesicle number in the collected SEC-fractions obtained from untreated (e), quiescent (f), and perpetuated (g) LX-2 (mean ± SD, n = 3). h–j Representative EAF4 fractograms of EV collected from untreated (h), quiescent (i), and perpetuated (j).