Fig. 5: Phylogenetic analysis and mapping of functional antibody clones against antigen C obtained from (i) single B-cells after bulk selection with antigen (cognate chain paired), (ii) AgSC and (iii) TBC libraries.

a Percent functional antibodies were identified from TBC (49 of total 196 clones or 25%) and AgSC (80 of total 203 clones or 39%) libraries and cognate paired Ag-selected single B cells (54 of total 75 clones or 72%). b Radial phylogenetic mapping of functional clones derived from AgSC and TBC libraries, and cognate paired Ag-selected single B cells (SBC) illustrates the diversity of clones from these platforms. The scale represents Juke–Cantor distance model as explained in the methods section. Cognate chain paired clones showed the greatest diversity followed by clones from AgSC and then by clones from TBC libraries. Sequences of clones from AgSC library were more closely related to those with cognate chain paired clones. There was very little overlap between TBC library derived clones with cognate chain paired clones.