Table 1 Relative number of cells at different stages of antigen specific B-cell isolation process for 5 antigens (A-E). The starting material were pools of cells from spleen and lymph nodes from 3 animals for each study.
Target antigen | Splenocytes (x106) | B cells (x106) | Total IgG+ B cells (x106) | Antigen-selected B Cells (x106) | Flow through cells (x106) | % Viability of antigen-selected B cells | % Antigen-selected Cells (of total B Cells) | % Antigen-selected Cells (of IgG+ B Cells) |
|---|---|---|---|---|---|---|---|---|
A | 304 | 67 | 19 | 0.13 | 18.3 | 94.5 | 0.19 | 0.66 |
B | 301 | 70 | 22 | 0.15 | 20.0 | 95.3 | 0.22 | 0.71 |
C | 285 | 75 | 25 | 0.72 | 23.7 | 96.7 | 0.95 | 2.92 |
D | 304 | 79 | 27 | 0.88 | 26.4 | 95.5 | 1.12 | 3.25 |
E | 294 | 83 | 24 | 0.21 | 23.1 | 94.7 | 0.25 | 0.87 |
