Fig. 2: Tse5-CT causes membrane depolarisation when expressed in P. putida.
Flow cytometry experiments were performed with P. putida cells harbouring pS238D1 empty vector (EV) as a negative control and plasmids directing the expression of Tse5-CT or sp-Tse5-CT. Each graph includes two positive controls: cells treated with a heat shock that results in cell permeabilization and cells treated with polymyxin B, which results in cell depolarisation. a Flow cytometry results showing healthy cell populations. Healthy cells are not marked by any fluorophore. b Flow cytometry data show depolarised cell populations. Depolarised cells are stained with DiBAC4(3). c Flow cytometry results show permeabilized cell populations. Permeabilized cells are stained with Sytox™ Deep Red. d Flow cytometry results show permeabilized and depolarised cell populations. All measurements were made in triplicate (n = 3 biological replicates). The graphs show the mean values and ±standard deviations (SD). The one-way ANOVA (Brown–Forsythe ANOVA test) with Dunnett´s T3 multiple comparisons test was used to determine whether there is a significant difference between the mean values of our independent groups (non-significant [ns] if p > 0.05, * if p ≤ 0.05, ** if p ≤ 0.01, *** if p ≤ 0.001).
