Table 1 Quantification of PhoA-LacZ enzymatic activities in permeabilised E. coli DH5α cells expressing Tse5-CT-PhoA-LacZ fusion proteins.

PhoA^a activities (A) (min⁻¹)

15(1)

8.8(2)

6.1(3)

8.58(4)

3.3(6)

LacZ^a activities (A) (min⁻¹)

36(6)

90(7)

85(10)

55(11)

100(2)

PhoA^b activities (%) (a.u.)

100(7)

58(1)

40(2)

56.5(2)

21(4)

LacZ^b activities (%) (a.u.)

36(6)

89(7)

85(9)

54(11)

100(2)

NAR^c

2.8(6)

0.65(6)

0.48(7)

1.0(2)

0.22(5)

Localisation

Periplasmic

TM

Cyto/TM?

TM

Cytosolic

Colour

Blue

Purple

Red-purple

Purple

Red