Fig. 2: ABPP of P. chrysosporium supernatants.
a Workflow of the analysis. P. chrysosporium suspension cultures were grown for 5 days on a minimal medium supplemented with beech wood chips. The solid material was filtered off, the filtrate lyophilized, and the residue was subjected to ABPP with the corresponding probes. b ABPP of SHs without (left panel, log2-fold change ≥2 compared to DMSO is indicated) or after pretreatment with 50 µM paraoxon (competition experiment, right panel) with 2 µM FP-alkyne after click chemistry (n = 4 biologically independent samples). Green dots indicate SHs. c ABPP of GHs without (left panel, log2-fold change ≥2 compared to DMSO is indicated) or after pretreatment with 20 µM KY358 (competition experiment, right panel) with 2 µM JJB111 (n = 4 biologically independent samples). Blue dots indicate GHs, while the red dot represents the DUF protein with four domains of unknown function Phchr2|3002168 with potential GH activity.
