Fig. 3: ABPP of P. chrysosporium substrate-bound fraction (SBF).
a Workflow of the SBF analysis. P. chrysosporium suspension cultures were grown for 5 days on a minimal medium supplemented with beech wood chips. The beech wood chips were isolated and substrate-bound proteins were isolated via 0.1% (w/v) dodecylmaltoside treatment. The obtained protein solution was lyophilized and the residue was subjected to ABPP with the corresponding probes. b ABPP of SHs without (left panel, log2-fold change ≥2 compared to DMSO is indicated) or after pretreatment with 50 µM paraoxon (competition experiment, right panel) and 2 µM FP-alkyne after click chemistry (n = 4 biologically independent samples). Green dots indicate annotated CEs. c ABPP of GHs without (left panel, log2-fold change ≥2 compared to DMSO is indicated) or after pretreatment with 20 µM KY371 (competition experiment, right panel) with 2 µM JJB111 (n = 3 biologically independent samples). Blue dots indicate annotated GHs.
