Table 1 Overview of the ABPP-identified enzymes from profiling the supernatant of P. chrysosporium suspension cultures grown on minimal medium supplemented with beech wood chips.

From: Identification of fungal lignocellulose-degrading biocatalysts secreted by Phanerochaete chrysosporium via activity-based protein profiling

Protein ID

Annotation

Used ABP

Log2 fold change

MW [kDa]

Predicted function

Phchr2|126075

CE1-CBM1

FP-alkyne

7.19

35.59

acetyl-xylan esterase

Phchr2|2912243

CE15

FP-alkyne

5.35

44.29

4-O-methyl-glucuronoyl methylesterase

Phchr2|2915237

GH5 subfamily 9

JJB111

10.71

46.55

1,4-β-endoglucanase

Phchr2|3024052

GH3

JJB111

8.55

86.85

1,4-β-xylosidase

Q66NB7; Phchr2|2985730

GH5 subfamily 6

JJB111

8.45

40.41

1,4-β-glucanase

Q9URP5;O74203

CBM1-GH3

JJB111

6.82

85.56

β-glucosidase

Phchr2|2895579

GH5 subfamily 9

JJB111

5.59

73.50

1,3-β-glucosidase

Phchr2|2990154

GH30 subfamily 3

JJB111

5.58

65.13

1,6-β-endoglucanase

Phchr2|3002168

DUF

JJB111

5.39

74.43

DUF1793, DUF4964, DUF4965, DUF5127

Phchr2|3004260

GH79

JJB111

4.99

48.99

β-glucuronidase

Phchr2|2981757

GH5 subfamily 5

JJB111

3.26

42.05

1,4-β-endoglucanase

Phchr2|2927412

GH74

JJB111

2.99

79.15

exo-1,3-β-xyloglucanase/reducing end-specific cellobiohydrolase

Phchr2|2916357

GH28

JJB111

2.52

53.53

galacturonase

W5ZNX7;C6H06; Phchr2|123909

GH16

JJB111

2.21

33.57

endo-1,3-β-glucanase

Phchr2|3006243

GH18, CBM5

JJB111

2.09

54.31

endo-1,3-β-glucanase

  1. Two SHs, 12 GHs, and one protein (DUF) with four DUFs with similarities to GHs were elucidated from FP-alkyne and JJB111 labeling, respectively.