Fig. 2: Structure determination of integrin β5 chimera with PAK4.
From: Molecular basis for integrin adhesion receptor binding to p21-activated kinase 4 (PAK4)
a Schematic of chimeric integrin β5-PAK4. The Linker region is a 12-residue -Ser-Ser-Gly- repeat. b–d P63 structure of integrin β5-PAK4 chimera at 3.5 Å (not submitted to PDB). e–g P41212 structure of integrin β5-PAK4D440N/S474E chimera (PDB ID: 7S47). Panels c and f show electron density maps that are unbiased by integrin β5, i.e. prior to adding integrin β5 to the model. Panels d and g show the final refined maps. Fobs–Fcalc maps contoured at +2.5σ (green) and −2.5σ (red). 2Fobs–Fcalc maps contoured at +1σ (blue) and +2σ (light blue).
