Fig. 2: Identification of intraocular EBOV and associated immunopathology.

All samples were collected post-mortem on day 99. a Gross pathology of both eyes, bisected, with dense cyclitic membrane present in left eye (OS). b Close-up of OS with red arrow pointing at dense membrane. c Schematic including quantification of EBOV GP genome equivalents (GEq), positive Goldmann–Witmer coefficient, and location of abnormal histopathology of the OS. d Abnormal histopathology OS. da Masson’s trichrome stained section showing a cyclitic membrane with nascent and organizing fibrosis infiltrated by fibroblasts, macrophages, plasma cells, and lymphocytes. db Low-power Masson’s trichrome stained section demonstrating iridocyclitis, vitritis, and cyclitic membrane. dc CD38⁺ plasma cells infiltrate the anterior retina and cyclitic membrane. dd CD8⁺ T lymphocytes within the vitreous body. de Masson’s trichrome stained section showing adhesion of the cyclitic membrane to the posterior lens capsule. df Plasma cells, macrophages, and lymphocytes infiltrate and expand the iris stroma. dg CD68⁺ macrophages infiltrate the vitreous body. dh CD4⁺ T lymphocytes within the cyclitic membrane. di Plasma cells and Mott cells in the ora serrata and anterior choroid. dj Macrophages and plasma cells in the retina (primarily ganglion cell and nerve fiber layer) and vitreous body. dk CD8⁺ T lymphocytes infiltrate and expand the inner retina as well as the vitreous body. dl CD38⁺ plasma cells in the inner retina. dm Masson’s trichrome stain showing macrophages and plasma cells in the posterior retina and vitreous body (detachment is artifactual). dn Plasma cells are present in the nerve fiber layer of the posterior retina. e Immune cell populations counted by flow cytometry. f Proinflammatory cytokine concentrations measured in the vitreous humor OS and OD of this infected animal and from an uninfected control animal.