Fig. 3: Mature NQs and eggs from the three colonies used in queen inhibition bioassays produce RNERO.
From: Identification of a queen primer pheromone in higher termites
Figure shows portions of 2D gas chromatograms of hexane washes of various available castes and life stages from colonies G–I, and of synthetic RNERO standard. Termites were sampled into identical amounts of solvent (30 µl/ind., 30 µl/approx. 50 eggs) directly from the colonies, except for young NQs, which were obtained in the bioassays and held in colony fragments for 9 h, 10, or 14 days. Figure depicts a portion of one modulation period (1–2 s) of the secondary column (BPX- 50) at the elution time of RNERO from the primary column (ZB5-MS). The shift in retention times of RNERO is due to the exchange of columns between analyses performed in 2021 (G, I) and 2022 (H).
