Fig. 6: Olfactory responses of female nymphs show a significant preference for RNERO over its opposite enantiomer and related sesquiterpenoids.
From: Identification of a queen primer pheromone in higher termites
a and b Graphs show EAG responses (amplitude values) of NY4♀ to air, RNERO, its opposite (S) enantiomer (SNERO) and two other structurally related sesquiterpenoids, (E,E)-α-farnesene and (E,E)-farnesol at doses of 50 ng (a) and 500 ng (b) of stimuli. The graphs show the means (±SD) of 15 independent stimulation series with randomized order of stimulations, related to responses to hexane stimulations; log2 scale is used. ANOVA test for both used doses revealed significant differences in antennal responses to different stimuli, with means ranked in the order: air < α-farnesene < farnesol < SNERO < RNERO (50 ng: F4,70 = 43.5, p < 10−4; 500 ng: F4,70 = 62.2, p < 10−4). Columns marked with different lowercase letters differed significantly at α = 0.05 when compared using Tukey’s multiple comparison test among all pairs of columns. Detailed test statistics are provided in the Supplementary Data 1 file. c EAG response traces showing one stimulation series using 500 ng of the compounds. d Structures of the tested compounds.
