Table 1 Sequence and activity of WCopW-derived AMPs.

Componud	Sequence^a													Net charge	MW	Minimum inhibitory concentrations (μM)^b
Componud	Sequence^a													Net charge	MW	MDR P. aeruginosa	MDR A. baumannii	MDR S. aureus	MDR E. faecalis
SCopW	NH₂	S	L	L	W	I	A	L	R	K	K		CONH₂	+4	1226.6	>20	>20	>20	>20
HCopW	NH₂	H	L	L	W	I	A	L	R	K	K		CONH₂	+5	1276.6	>20	>20	>20	>20
WCopW	NH₂	W	L	L	W	I	A	L	R	K	K		CONH₂	+4	1325.7	20	5	5	10
WCopW1	NH₂	W	L	L	W	I	A	L	R	K	K	R	CONH₂	+5	1481.9	>20	5	2.5	5
WCopW3	NH₂	w	l	l	w	i	a	l	r	k	k	r	CONH₂	+5	1481.9	5	2.5	1.25	2.5
WCopW5	NH₂	w	l	l	w	i	g	l	r	k	k	r	CONH₂	+5	1467.9	10	1.25	1.25	2.5
WCopWK	NH₂	w	l	k	w	i	g	l	r	k	k	r	CONH₂	+6	1482.9	>20	>20	>20	>20
WCopWE	NH₂	w	l	e	w	i	g	l	r	k	k	r	CONH₂	+4	1483.8	>20	10	>20	>20
WCopWY	NH₂	w	l	y	w	i	g	l	r	k	k	r	CONH₂	+5	1517.9	20	1.25	10	2.5
WCopWV	NH₂	w	l	v	w	i	g	l	r	k	k	r	CONH₂	+5	1454.8	10	1.25	2.5	2.5
WCopWF	NH₂	w	l	f	w	i	g	l	r	k	k	r	CONH₂	+5	1502.9	10	1.25	2.5	2.5
Melittin															2846.5	>20	1.25	1.25	1.25

^aThe sequence of the coprisin orthologous α-helical region-derived short AMP, WCopW, and its derivatives. Lower case letters indicate d-form amino acids; capital letters, l-form. Hydrophilic position-preferring residues are remarked as bolded letters because of their critical role in matching the preferred membrane position profile.
^bThe minimal inhibitory concentrations (MICs) were determined in cation-adjusted Mueller–Hinton broth containing 10 mg/l Mg²⁺ and 50 mg/l Ca²⁺ under CLSI conditions. When considering the molecular weight of AMPs (≤1.52 kDa), a MIC of 2.5 μM (≤3.8 μg/ml) (bold letters) could be considered as the “breakpoint” MIC (i.e., <4 μg/ml), but a MIC of 1.25 μM (≤1.9 μg/ml) (bold and underlined letters) is a more confident “breakpoint” MIC (10). Melittin was used as a control because it is one of the most powerful nonclinical AMPs (Table 3 and Supplementary Table S1).

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