Fig. 6: Causal link between offline activity during post-learning NREM sleep and long-term storage of STM trace.
From: A short-term memory trace persists for days in the mouse hippocampus
a Diagram of AAV injection and CA1 optic fiber placement. b Representative CA1 sections showing ArchT-eYFP (Left: test group) and eYFP (Right: control group) protein expressions (scale bar = 100 μm; dashed line: guide cannula location). c Experimental design for optogenetic silencing of STM trace storage using NOL two-training paradigm during post-learning NREM sleep. d Example from one mouse showing 2-hour EEG/EMG recordings with laser ON during NREM state only (top), and example of EEG/EMG traces for NREM, REM, and awake states (bottom) (see Methods section for details). Exploration preference for each object during the first (e) and the second (f) training sessions for ArchT (n = 8) and eYFP (n = 10) groups. g Exploration preference for each object during the test session in ArchT (n = 8) and eYFP (n = 10) groups. h Duration of NREM sleep (laser ON) in ArchT (n = 8) and eYFP (n = 10) groups. Comparisons were made using wilcoxon signed rank sum test (e) and paired student’s t-test for comparing object A and object A* in the same group, and unpaired Student’s t-test for comparing object A* within different groups (f, g) and wilcoxon rank sum test (h); ns not significant (P > 0.05), **P < 0.01, ***P < 0.001. Data are presented as the mean ± SEM.
