Fig. 2: Magic-angle spinning solid-state NMR analysis of cell-free synthesized amyloid fibrils. | Communications Biology

Fig. 2: Magic-angle spinning solid-state NMR analysis of cell-free synthesized amyloid fibrils.

From: Cell-free synthesis of amyloid fibrils with infectious properties and amenable to sub-milligram magic-angle spinning NMR analysis

Fig. 2

a Primary sequence of HET-s (in orange), NMR-active isotope (13C, 15N)-labeled in the CF-GVI-HET-s sample are colored in blue. b Structure of the repeats R1 and R2 of HET-s PFD amyloid fibrils, G, V, and I residues are shown as spheres with carbon atoms colored in blue. c 2D hNH spectra of R-HET-s (orange) and CF-GVI-HET-s (blue) recorded at 100 kHz MAS, d 2D hCH experiments on R-HET-s (orange) and CF-GVI-HET-s (blue) recorded at 100 kHz MAS. e 1D 1H trace extracted from 2D hCH spectrum for residue G271 and V264.

Back to article page