Table 1 Effect of temperature on the relevant mechanical parameters listed on the first row.

From: The force of the myosin motor sets cooperativity in thin filament activation of skeletal muscles

 

Temperature (°C)

T0,4.5 (kPa)

s0 (nm)

F0 (pN)

nH

pCa50

soleus fibre

      

control

      
 

12.3 ± 0.1 (14)

127 ± 5

3.16 ± 0.12

1.71 ± 0.06

1.97 ± 0.07

6.38 ± 0.05

 

17.3 ± 0.1 (4)

148 ± 12

3.78 ± 0.25

2.04 ± 0.14

2.42 ± 0.09

6.54 ± 0.06

 

25.2 ± 0.1 (4)

165 ± 12

4.53 ± 0.20

2.45 ± 0.11

3.15 ± 0.13

6.55 ± 0.03

 

35.5 ± 0.1 (5)

183 ± 3

5.34 ± 0.32

2.59 ± 0.19

3.09 ± 0.20

6.67 ± 0.09

1 µM OM

      
 

12.2 ± 0.1 (5)

69 ± 3

1.53 ± 0.05

0.83 ± 0.06

0.79 ± 0.09

7.17 ± 0.09

 

25.2 ± 0.1 (5)

117 ± 14

2.75± 0.34

1.48 ± 0.18

0.96 ± 0.13

7.21 ± 0.10

 

35.6 ± 0.1 (5)

134 ± 14

3.11 ± 0.30

1.68 ± 0.16

1.17 ± 0.08

7.16 ± 0.08

psoas fibre

      
 

6.0 ± 0.1 (4)

135 ± 6

1.71 ± 0.11

2.90 ± 0.18

3.51 ± 0.22

5.89 ± 0.05

 

12.1 ± 0.1 (12)

219 ± 11#

2.86 ± 0.16#

4.87 ± 0.27

3.92 ± 0.21

6.00 ± 0.13

 

25.0 ± 0.1 (6)

317 ± 14#

4.13 ± 0. 19#

7.02 ± 0.32

4.81 ± 0.22

6.46 ± 0.06

  1. Data for soleus fibres in control and in the presence of 1 µM OM. For comparison also data for psoas fibres are reported (from ref. 33 and new data). Data are mean ± SEM. In brackets the number of fibres used for each temperature. # mean calculated by pooling data from new experiments with data from ref. 33. In each of the three cases the statistical significance of the temperature effect is tested with one-way ANOVA as detailed in Supplementary Table 2. The soleus values in control of F0 and nH at 35.6 °C are not significantly different from the corresponding psoas values at 6.0 °C (P = 0.24 and 0.16 respectively).
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