Table 1 Microglia specificity of AAV vectors in different brain regions.

AAV construct		Cerebral cortex	Striatum	Cerebellum	n
Promoter	miR target	Cerebral cortex	Striatum	Cerebellum	n
PGK	miR-9.T	12.0 ± 3.7 (319/2656)	7.4 ± 3.4 (191/2576)	34.4 ± 10.8 (901/2620)	5
Iba1	–	2.1 ± 1.3* (59/2791)	68.8 ± 9.6** (1808/2628)	85.7 ± 5.5 (2244/2618)	5
Iba1	miR-9.T	27.3 ± 2.4**^† (753/2760)	94.0 ± 2.0**^† (2663/2833)	100 ± 0*** (2726/2726)	5
Iba1	miR-9.T miR-129-2-3p.T	86.5 ± 5.6**^†‡ (2750/3180)	99.6 ± 0.5**^† (3127/3140)	100 ± 0*** (2712/2712)	5

Data are presented as mean ± s.d. (%). Numbers in parentheses indicate the number of cells counted (GFP- and Iba1-positive cells/GFP-positive cells). Comparison of microglial specificity between AAV groups one week after the injection was assessed as follows: cerebral cortex, one-way ANOVA: F_(3,16) = 540.425, P < 0.001; Bonferroni post hoc analysis: *P = 0.003, **P ≤ 0.001 for vs. PGK.miR-9.T, ^†P ≤ 0.001 for vs. Iba1, ^‡P ≤ 0.001 for vs. Iba1.miR-9.T; striatum, one-way ANOVA: F_(3,16) = 330.221, P < 0.001; Bonferroni post hoc analysis: **P ≤ 0.001 for vs. PGK.miR-9.T, ^†P ≤ 0.001 for vs. Iba1; cerebellum, Kruskal–Wallis test, Kruskal–Wallis statistic = 18.348, P < 0.001; post hoc Dunn’s analysis with Bonferroni adjustment for multiple comparisons: ***P = 0.002 for vs. PGK.miR-9.T. AAV adeno-associated virus, Iba1 ionized calcium-binding adapter 1, miR microRNA, n number of mice per group, PGK phosphoglycerate kinase 1.

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