Fig. 1: Identification of human-rhesus cross-reactive antibodies. | Communications Biology

Fig. 1: Identification of human-rhesus cross-reactive antibodies.

From: Single cell multi-omic reference atlases of non-human primate immune tissues reveals CD102 as a biomarker for long-lived plasma cells

Fig. 1

a Assay scheme for multiplexing cells from different tissues prior to input into high-throughput flow cytometry-based antibody screen (LEGENDScreen). Briefly, cells from each tissue are labeled with a unique CD45-fluorophore combination prior to being mixed and stained with a panel of antibodies against lineage markers. Cells from each tissue are demultiplexed during analysis (right panel). b Histograms of indicated marker expression on lymphocytes for LEGENDScreen assay. Assay marker is in red and isotype control is in gray. c Histograms of tissue-specific expression patterns of indicated markers. Cells from bone marrow, lymph node, and PBMC are colored orange, red, and green, respectively. d Venn Diagram depicting shared and unique expression patterns of the 112 human-rhesus cross-reactive antibodies identified. Overlaps between circles indicate shared expression between tissue compartments. e Heatmap displaying expression patterns of the 112 human-rhesus cross-reactive antibodies identified across the tissues compartments assessed.

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