Fig. 2: Multi-modal single-cell atlas of rhesus macaque immune tissues. | Communications Biology

Fig. 2: Multi-modal single-cell atlas of rhesus macaque immune tissues.

From: Single cell multi-omic reference atlases of non-human primate immune tissues reveals CD102 as a biomarker for long-lived plasma cells

Fig. 2

a Experimental design for rhesus single-cell data generation. Tissues were collected from healthy rhesus macaques. Single-cell suspensions were isolated from fresh blood, lymph nodes, and bone marrow. To enable profiling of cell surface proteins, cells were labeled with a panel of 124 oligo-conjugated antibodies before being subjected to droplet-based single-cell RNAseq on the 10x Chromium platform. Diagram created with BioRender.com. b Table of animal subject metadata for NHP included in single-cell multi-omic analysis and number of cells recovered across tissue compartments assessed. NA indicates data not available. c UMAP visualization of cells derived from NHP BM, LN, and PBMC. Cells are colored by major cell lineage. d Left panel: UMAP visualization of cells by tissue of origin. Right panel: Distribution of major cell lineages by tissue. e Expression of canonical PC genes at the RNA level. Expression is visualized as the kernel density estimate for each RNA. f Proportions of PC across the three tissues profiled.

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