Fig. 5: CD102 and CD31 expression is conserved across species. | Communications Biology

Fig. 5: CD102 and CD31 expression is conserved across species.

From: Single cell multi-omic reference atlases of non-human primate immune tissues reveals CD102 as a biomarker for long-lived plasma cells

Fig. 5

a Left panel: Representative histograms of CD31 and CD102 expression on bulk lymphocytes or CD138 + plasma cells on cells from mouse bone marrow. CD138 + plasma cells were previously gated as live, singlets, CD4−CD8−F4/80−NK1.1−IgD−. Lymphocytes were previously gated as live singlets. Right panel: Quantification of CD31 and CD102 expression on bulk lymphocytes or CD138 + plasma cells on cells from mouse bone marrow. Error bars represent standard error of the mean. n = 5 biologically independent animals. b Top panel: Representative flow plot of plasma cell gates and CD102 and CD31 expression on plasma cells from human bone marrow cells. Bottom panel: Representative flow plot of frequency of plasma cells within CD102 + CD31 + cells. c Representative images of ELISPOT wells coated with either anti-IgG or anti-IgA. CD102 + CD31 + or CD102−CD31− cells from human bone marrow were plated in duplicate and serial diluted (2-fold) down the plate. Loadings for the top dilution are indicated. d Quantification of ELISPOT results depicting the number of ASCs identified per million input cells for both total-IgG and total-IgA secreting cells. Error bars represent standard deviation from the mean. n = 4 biologically independent human subjects.

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