Fig. 1: The enzymatic reactions catalyzed by 2-HIS and 2-HID.
From: The protein conformational basis of isoflavone biosynthesis
2-HIS catalyzes hydroxylation and aryl-ring migration of flavanones (e.g., liquiritigenin and naringenin) to convert them to 2-hydroxyisoflavanones, and 2-HID then catalyzes dehydration of 2-hydroxyisoflavanones to yield the final isoflavone products, e.g., daidzein or genistein.
