Fig. 6: The MST dose-response curves and SPR sensorgrams. | Communications Biology

Fig. 6: The MST dose-response curves and SPR sensorgrams.

From: In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa

Fig. 6

The dose-response curve between a RlpA-Δ32 and MltF2, b RlpA-Δ32 and PBP1b, c RlpA-Δ32·MltF2 and PBP1b. RlpA-Δ32 at a concentration of 5 nM is set as the fluorescently-labeled target throughout each MST run (ligand units are in molar). d RlpA-Δ32 and MltF2 SPR sensorgram, with concentrations of the latter decreasing in two-fold dilution. e RlpA-Δ32 and PBP1a SPR sensorgram, with concentrations of the latter decreasing at five-fold dilutions. f RlpA-Δ32·MltF2 and PBP1a SPR sensorgram, with concentrations of the latter decreasing at two-fold dilutions. RlpA-Δ32 is set as the immobilized ligand on the surface of the carboxymethylated dextran chip throughout the SPR runs. The response is measured in response units (RU). Concentration units for SPR experiments are given in μM. The baseline is corrected to zero. a–c Data were presented as means ± SD from triplicate experiments. View Supplementary Data for source data.

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