Fig. 2: Evaluation of selected antibodies.
From: Evaluation of commercially available glucagon receptor antibodies and glucagon receptor expression
a Immunohistochemical staining of liver tissue from either the glucagon receptor wildtype (Gcgr+/+) or glucagon receptor knockout (Gcgr−/−) female mice, 8 weeks of age, using the 12 GCGR antibodies (1:100). Staining intensity scores between 0 and 3 of liver tissues from both the Gcgr+/+ and Gcgr−/− mice are presented, the higher the score, the more receptor-antibody binding. X40, scale bar = 50 µm. The observed differences in background in the slides are suspected to be related to hematoxylin staining. b Uncropped western blots of transfected cells with either human glucagon receptor (GCGR) cDNA or mouse GCGR cDNA. Western blotting performed with selected antibodies no. 4, 10 and 11 (1:500). Uncropped western blots of a mock transfection showing a negative staining with antibody no. 11. and a western blot with loading control GAPDH are shown in Fig. S3.
