Fig. 3: GCGR expression using immunohistochemistry.
From: Evaluation of commercially available glucagon receptor antibodies and glucagon receptor expression
a Immunohistochemical staining of various tissues from glucagon receptor wildtype (Gcgr+/+) and knockout (Gcgr−/−) female mice, 8 weeks of age, using the selected antibody no. 11 (1:100). Positive immunostainings were found in following tissues: heart, pancreas, kidney, liver, gastric mucosa, duodenum, ileum, brown adipose tissue (BAT), and the cortex of the adrenal gland, with a corresponding negative staining of Gcgr−/− tissue. No immunostaining was observed in the colon, white adipose tissue (WAT) and muscle tissue. Mouse liver, kidney, pancreas, WAT, colon, ileum, duodenum, adrenal gland, X100, scale bar = 30 µm. BAT, heart, muscle, gastric mucosa, X75, scale bar = 40 µm. See also Fig. S5 for immunohistochemical staining of premature adipocytes. b Antibody staining of healthy human renal tissue from autopsy using the selected antibody no. 11 (1:100). Cells within the proximal tubule (PT) were not stained, whereas cells in the distal tubules (DT) showed immunoreactivity toward the glucagon receptor (GCGR). X100, scale bar = 100 µm. c Co-staining of pancreatic mouse tissue, from Gcgr+/+ female mice 8 weeks of age, with antibody no. 11 (1:100) and either glucagon (Scbt/Cat no. sc-514592), insulin (mybiosource/cat no. MBs448113), or somatostatin (Everestbiotech/cat no. EB11971). DAPI staining of nuclei. X200, scale bar = 20 µm. Bar insert = 6 µm, X400. Arrows indicate cells that were co-stained.
