Fig. 4: Comparison of glucagon receptor expression by antibody and antibody-independent approaches.
From: Evaluation of commercially available glucagon receptor antibodies and glucagon receptor expression
Microphotographs of the liver, kidney, adrenal gland, duodenum, pancreas, muscle tissue, gastric mucosa, heart, colon mucosa, spleen, and adipose tissue of female C57BL/6JRj mice, 13 weeks of age, that received an intravenous injection of 3 pmol 125I-Glucagon or 5 nmol of non-radioactive glucagon in combination with 3 pmol of 125I-Glucagon to test for specific binding. Autoradiography of mouse liver, kidney and pancreas showed high density of grains in the tissue, which was attenuated in mice receiving both labelled and non-labelled glucagon. Liver, adrenal gland, pancreas, gastric mucosa, colon mucosa, and fat tissue, X100, scale bar = 50 µm. Kidney, duodenum, muscle, heart muscle, and spleen, X70, scale bar = 70 µm.
