Table 1 Overview of glucagon receptor expression by antibody and antibody-independent approaches.
From: Evaluation of commercially available glucagon receptor antibodies and glucagon receptor expression
Presence of GCGR using IHC staining with antibody no. 11 | Presence of GCGR using a radiolabeled ligand | Presence of GCGR using RNA-sequencing | Studies located GCGR using immunostaining | Studies located GCGR using a radiolabeled ligand | |
|---|---|---|---|---|---|
Liver | Yes | Yes | Yes (scRNA-sequencing indicates specific location primarily within hepatocytes) | Previously located in rats, using monoclonal antibody34 | |
Kidney | Yes | Yes | Yes (scRNA-sequencing suggests specific location within collecting and distal tubule cells) | Previously located in rats, using monoclonal antibody34 | |
Pancreas | Yes | Yes | Yes (scRNA-sequencing indicates specific location primarily within Beta-cells) | Previously located in rats and INS1 cells25 | Previously located in In rats and INS1 cells25 |
Duodenum | No | No | No | ||
Ileum | Yes | – | No | Previously located in mice, small intestine51 and dog intestinal smooth muscle cells56 | |
Heart | Yes | No | No | Previously located in mice51 | |
Fat Tissue | Yes (Preadipocytes and BAT) | No | No | Previously located in rats, using monoclonal antibody34 | Previously located in mice51 |
Adrenal gland | Yes (cortical part) | No | No | ||
Gastric mucosa | Yes | No | No | Previously located in mice51 | |
Colon | No | No | No | Previously located in rats and INS1 cells25 | Previously located in rats and INS1 cells25 |
Nerve tissue | – | – | Yes |
