Fig. 2: Enzyme activity analyses of Ht-R15P isomerase and Hx-RbsK proteins with HPLC.

a In the presence or absence of Ht-R15P isomerase enzyme, the generation of RuBP from R15P was investigated. Black, pink, blue, and green lines indicate the reaction product without the enzyme, that with the enzyme, 20 mM R15P standard compound, and 10 mM RuBP standard compound, respectively. b In the presence or absence of the Hx-RbsK protein, the generation of R15P from R1P was examined. Black, pink, blue, and green lines indicate the reaction product without the enzyme, that with the enzyme, 10 mM R1P standard compound, and 20 mM R15P standard compound, respectively. c Conversion of R1P with the Hx-RbsK and Ht-R15P isomerase proteins was examined. After the kinase reaction by Hx-RbsK, the isomerase reaction by Ht-R15P isomerase was carried out. The reaction product was analyzed by HPLC. Pink and black lines indicate reaction products with and without Ht-R15P isomerase in the second reaction, respectively. Compounds separated with a column were monitored with a differential refractive index detector.