Fig. 8: In vivo experiments identify the inverse correlations of IL-1β with STAT3 in aorta of mice. | Communications Biology

Fig. 8: In vivo experiments identify the inverse correlations of IL-1β with STAT3 in aorta of mice.

From: Macrophages regulate vascular smooth muscle cell function during atherosclerosis progression through IL-1β/STAT3 signaling

Fig. 8: In vivo experiments identify the inverse correlations of IL-1β with STAT3 in aorta of mice.

a Western blot assays illustrate the effect of adeno-associated virus-sgIL-1β (AAV-sgIL-1β) on the expression of main SMC subsets transcription factors in vivo. Quantifications of b p-STAT3, c vascular smooth muscle cell (VSMC) marker (α-SMA), d macrophage marker (CD68) in aorta of mice from each group. Representative immunofluorescence staining of p-STAT3 (yellow) expressing plaque macrophage-like VSMCs in ApoE−/− mice at e 8 weeks, f 16 weeks, and treated with (g) AAV-sgIL-1β fed HFD. Eighteen ApoE−/− mice were randomly grouped into three groups, including high-fat diet (HFD) treated 8 weeks (n = 6), 16 weeks (n = 6), and AAV-sgIL-1β (n = 6) mice. Data are presented as mean ± SE. Mann–Whitney test: *P < 0.05.

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