Fig. 4: Mass responses to drugs with various mechanisms of action.

Therapies with different mechanisms of action (MOA) induce different characteristic mass response signatures. As depicted in (a) these MOA signatures capture drug effects that can be loosely classified into three general categories, including cell cycle arrest, metabolic disruption, or loss of structural integrity. b Schematic demonstrations of the changes in mass distributions between a control population (gray fill, dotted line) and a drug-treated population (red fill, solid line) caused by G1 arrest, G2 arrest, catabolic skew, anabolic skew, or cell membrane loss. c Example experimental mass distribution changes in response to drug treatment corresponding to each schematic result presented directly above in (b). d Mass response measurements collected with three different instruments (individual points with error bars corresponding to the 95% confidence interval of EMD for each system defined by 5000 random samples of 2500 cells), corresponding to the experimental data presented directly above in (c).