Fig. 3: Iowa mosquito surveillance data confirm the natural diapause timeline.

Mosquito surveillance from central Iowa display mosquito populations trends that examine general population abundance (a) or represent only reproductive populations (b). Data in (a) represent Cx. pipiens group abundance measured using New Jersey Light Traps (NJLT) from 14 sites in central Iowa, while data in (b) display Gravid trap data from 16 sites in central Iowa that monitor reproductive female Cx. pipiens populations. For both a and b, collections were performed from 2016–2021 from weeks 20 to 40. Individual gray dots represent yearly trap index averages by week, with the blue (NJLT) or red (Gravid) lines representing the loess-smoothed mean and 95% confidence intervals (gray-shaded area). The approximate period of diapause receptivity (as defined by our semifield experiments) is displayed by the red gradient.