Fig. 5: Kinetochore proximal phosphosites reduced after AB1 inhibition of the kinetochore kinases CLK1/CLK2.

a Spatially referenced proximity phosphoproteomics was used as in Fig. 2 but with AB1 treatment from 0 h after synchronisation release. b Principal component analysis (PCA) of phosphopeptides quantified by spatially referenced proximity phosphoproteomics. PCA was performed on label-free quantified phosphopeptides with an ANOVA q value <0.1. c Heatmap of 191 proximal phosphosites. Each row is a phosphosite proximal to KKT3 or the spatial reference BDF5 in at least one timepoint at 5% FDR. Phosphosites are clustered by label-free abundance similarity across the samples. Samples are also clustered by similarity. d Label-free quantification of all phosphopeptides identified in AB1-treated vs. DMSO-treated parasites at 4 h after AB1 treatment and synchronisation release (top) and 8 h (bottom). Significantly changing phosphosites after AB1 treatment are either non-proximal to KKT3 (pink) or proximal to KKT3 (red). The dashed line indicates 5% FDR, n = 5.