Fig. 3: Filament network and filament-membrane orientation.
From: A network of mixed actin polarity in the leading edge of spreading cells
a An x-y slice through a cryo- tomogram (left) and its rendered isosurface view of a lamellipodium protrusion. Actin filaments (gray), cell membrane (turquoise), membrane receptors (purple), and cell components (magenta). Scale bar: 300 nm. b The orientation of filaments with respect to the membrane was measured in 47 tomograms at the membrane proximal area of the cells (0–40 nm away from membrane) and distal area (360–400 nm away from membrane). The dark gray region represents filaments that contain vectorial components with the barbed end towards the membrane. c The boxplots showing the proportion of the different filament directionalities with respect to the membrane, with 1 SD and 1.96 SEM in whiskers (N = 47 tomograms).
