Fig. 6: L1CAM triggers sphere formation in primary fallopian tube cell lines.

a Western blot analysis of primary fallopian tube cell lines transfected with L1CAM or a control vector and analyzed for L1CAM and GAPDH (housekeeping gene) expression. b Bright field images of primary fallopian tube cell lines from a. Scalebar represents 200 µm. c Quantification of area in µm² of spheres shown in b. d (right panel) Fluorescent images representing FT237 cells transfected with a L1CAM or (left panel) a control vector. Dot plot depicting the ratio between living and dead cells, assessed by measuring the ratio between calcein (green) and ethidium bromide (red) intensity in FT237 cells transfected with L1CAM or a control vector after culturing under 3D and serum free conditions. Scalebar represents 100 µm. (e, left panel) Dot plot showing sphere size of FT237 cells that was analyzed after transfection with control vector or L1CAM with the addition of either IgG antibody or the anti L1-9.3 antibody. (e, right panel) Fluorescent (calcein, green) images representing FT237 cells transfected with control vector (left) or L1CAM with the addition of either IgG antibody or the anti L1-9.3 antibody. Scalebar represents 250 µm. Three independent experiments were performed (c, d). P-values were calculated with an unpaired two-sided t-test. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001. e ANOVA and Dunnett multiple comparison tests were used to calculate significance.