Fig. 1: Multi-layer chain length control strategy. | Communications Biology

Fig. 1: Multi-layer chain length control strategy.

From: Engineering Pseudomonas putida KT2440 for chain length tailored free fatty acid and oleochemical production

Fig. 1

The chain length of the final product is influenced by three layers of control. The use of an engineered acyl-ACP thioesterase favors the production of medium-chain FFAs in layer 1. Selectively knocking out CoA ligases with medium-chain FFA substrate preference prevents the catabolism of medium-chain FFAs while still allowing for catabolism of long-chain FFAs in layer 2. The use of a medium-chain fatty acid methyltransferase further influences the final chain-length profile in layer 3.

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