Fig. 3: R1-6 photoreceptor cluster identification and Zasp66 is a novel marker for adult photoreceptor cells. | Communications Biology

Fig. 3: R1-6 photoreceptor cluster identification and Zasp66 is a novel marker for adult photoreceptor cells.

From: Single cell RNA sequencing of the adult Drosophila eye reveals distinct clusters and novel marker genes for all major cell types

Fig. 3

a R1-6 cell clusters in Seurat UMAP cluster plots of 7-day-old adult eyes are colored red. b–d FeaturePlots showing gene expression of ninaE (b), oc (c), and Zasp66 (d). All cells expressing oc and Zasp66 were brought to the front in FeaturePlots (c), (d). e Schematic of an ommatidium where the bracket and red dotted line mark the focal plane of the views in (g) and (h), respectively. f ViolinPlot of log-normalized ninaE expression in all cell types. g Tangential view of a 7-day-old Zasp66-T2A-Gal4 > UAS-mCherry-nls adult eye costained with mCherry and Elav in red and blue, respectively. h Coronal view of 7-day-old Zasp66-T2A-Gal4 > UAS-mCherry-nls adult eye costained with Elav and Ct. Solid white bracket marks the R1-7 nuclei and dashed white bracket marks R8 nuclei. Scale bars: 10 μm.

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