Fig. 5: Pigment cell cluster identification and santa-maria and wrapper are two novel markers for adult pigment cells. | Communications Biology

Fig. 5: Pigment cell cluster identification and santa-maria and wrapper are two novel markers for adult pigment cells.

From: Single cell RNA sequencing of the adult Drosophila eye reveals distinct clusters and novel marker genes for all major cell types

Fig. 5

a Pigment cell clusters are colored in red in Seurat UMAP cluster plots of 7-day-old adult eyes. b–e FeaturePlots showing the expression of w (b), Pdh (c), santa-maria (d), and wrapper (red arrow, (e). All cells expressing w, santa-maria and wrapper were brought to the front in FeaturePlots (b), (d), and (e). f ViolinPlot of log-normalized Pdh expression in all cell types. g Tangential view of a 7-day-old adult eye prepared from a santa-maria-T2A-Gal4 > UAS-mCherry-nls animal and costained for mCherry and Elav. The green dotted hexagon marks the border of one ommatidium. h Coronal view of a 7-day-old adult eye from a santa-maria-T2A-Gal4 > UAS-mCherry-nls animal. i Coronal view of immunofluorescence images of 7-day-old adult eyes from a wrapper-T2A-Gal4 > UAS-mCherry-nls animal costained with Elav. j, k Tangential and coronal views of immunofluorescence images of 7-day-old adult wrapper-T2A-Gal4 > UAS-mCherry-nls eyes costained with Ct. Scale bars: 10 μm. l Schematic of an ommatidium with the red dotted boxes and black brackets marking the focal planes shown in (g–k).

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