Fig. 1: Proteomics workflow in THRSP transgenic mice. | Communications Biology

Fig. 1: Proteomics workflow in THRSP transgenic mice.

From: Hippocampal dentate gyri proteomics reveals Wnt signaling involvement in the behavioral impairment in the THRSP-overexpressing ADHD mouse model

Fig. 1

a Confirmation of transgenic backgrounds in mice was performed via DNA electrophoresis (Genotyping) and qRT-PCR (Thrsp mRNA expression) analyses (n = 18 mice/group, two-tailed, paired t test: (t = 11.9, df = 17, P < 0.001)). Thrsp mRNA was not detected in THRSP KO mice. Subsequently, mice were exposed to the Y-maze test to reconfirm inattention in THRSP OE mice when compared to WT and THRSP KO mice (n = 18 mice/group: one-way ANOVA, F (2, 51) = 51.7, P < 0.001). THRSP OE mice show sustained inattention and memory impairments. Values are presented as the mean ± standard error of the mean (SEM). b Following the confirmation of behavior in mice, hippocampal DG were prepared for subsequent proteomics analysis. c Summary of identified DEPs in mouse hippocampal DG. THRSP/Thrsp thyroid hormone-responsive protein, OE overexpressing, DG dentate gyrus, DEPs differentially expressed proteins. The images used in (b) was created with BioRender.com.

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