Fig. 4: Canonical and noncanonical Wnt pathway elements expressions in the mouse hippocampal DG. | Communications Biology

Fig. 4: Canonical and noncanonical Wnt pathway elements expressions in the mouse hippocampal DG.

From: Hippocampal dentate gyri proteomics reveals Wnt signaling involvement in the behavioral impairment in the THRSP-overexpressing ADHD mouse model

Fig. 4

qRT-PCR analyses of a canonical and b noncanonical Wnt ligands (n = 6 mice/group; a two-way ANOVA, Strain: F (2, 45) = 3.24, P = 0.048; Gene targets: F (2, 45) = 3.58, P = 0.036; Strain × Gene targets: F (4, 45) = 3.41, P = 0.016; b two-way ANOVA, Strain: F (2, 44) = 1.35, P = 0.269; Gene targets: F (2, 44) = 3.53, P = 0.038; Strain × Gene targets: F (4, 44) = 0.626, P = 0.647). The mRNA expression levels of c canonical and d noncanonical Wnt inhibitors (n = 6 mice/group; c two-way ANOVA, Strain: F (2, 133) = 1.70, P = 0.186; Gene targets: F (8, 133)  = 9.61, P < 0.001; Strain × Gene targets: F (16, 133) = 7.31, P < 0.001; d two-way ANOVA, Strain: F (2, 60) = 5.16, P = 0.009; Gene targets: F (3, 60) = 0.754, P = 0.524; Strain × Gene targets: F (6, 60) = 1.99, P = 0.081). mRNA levels of e Wnt receptors, f canonical Wnt co-receptors, and g noncanonical Wnt co-receptors. e Two-way ANOVA, Strain: F (2, 24) = 11.18, P < 0.001; Gene targets: F (1, 24) = 6.67, P = 0.0.16; Strain × Gene targets: F (2, 24) = 0.182, P = 0.835; f two-way ANOVA, Strain: F (2, 59) = 5.33, P = 0.007; Gene targets: F (3, 59) = 2.10, P = 0.110; Strain × Gene targets: F (6, 59) = 4.76, P < 0.001; g two-way ANOVA, Strain: F (2, 45) = 1.64, P = 0.205; Gene targets: F (2, 45) =  15.2, P < 0.001; Strain × Gene targets: F (4, 45) = 0.546, P = 0.703). Values are presented as the mean ± standard error of the mean (SEM). Data show differential expression of canonical and noncanonical Wnt pathway elements. DG dentate gyrus, qRT-PCR real-time quantitative reverse transcription PCR.

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