Fig. 2: Comparison of in-vial and tip-based IMAC for phosphopeptide enrichment.
From: A streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics
a The processing time for in-vial and tip-based IMAC. The numbers of identified phosphopeptides (b), phosphoproteins (c) and the specificity of phosphopeptide enrichment (d) for unlabeled and TMT-labeled peptides from MCF-7 cells using the in-vial and tip-based IMAC are shown. Sample amount: 150 μg of peptides. The error bars represent the standard deviation (n = 3).
