Fig. 6: Phosphoproteome analysis of LCM-dissected human spleen tissue voxels.
From: A streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics
a The optical image and the TMT experiment design of the white (TMT132C, TMT131N, TMT133C, and TMT134N), and red pulp regions (TMT130C, TMT131N, TMT131C, and TMT132N), and the number of quantified phosphopeptides from IMAC eluent and proteins from IMAC flow-through (FT). b The overlap of quantified proteins between IMAC eluent and FT. c PCA of the phosphoproteome data. d Volcano plot (t-test, n = 8 for each condition; s0 = 1 and FDR = 0.05% were used as cut-off values) shows significantly changed phosphopeptides in white pulp and red pulp. e The significantly altered phosphorylation sites in the MSFA1 (CD20) proteins. f The annotated pathway for the significantly up-regulated phosphorylation peptides in white or red pulp regions by STRING. g The quantitation correlation between global and phosphoproteome. h Known signaling transduction for triggering apoptosis in activated B cells.
