Fig. 5: Zyxin overexpression increases abundance of f-actin and focal adhesions.

a–d To compare stress fibers and focal adhesions, D3/E14 transfected with mCherry vector control (ctrl) and mCherry-Zyxin (zyxin O/E) were immuno-stained with Phalloidin-Alexa 488 (a, b) and vinculin (c, d). White box areas were further amplified and shown as the zoomed in images. White arrows denoted f-actin and focal adhesion. Z-stacked images with maximum intensity projection were shown. Representative images from three biological repeats were shown. Scale bar: 30 μm for original images, 5 μm for zoomed in images. e projected cell area, f integrated actin intensity, g number of focal adhesions per cell, h projected focal adhesion areas were statistically analyzed and plotted. Quantified data were from three independent experiments (N = 90). Mann–Whitney U-test was used to test the differences between the control and zyxin overexpression groups. In the box and whisker plots, the center line is the median, the box-bounds are the 25th and 75th percentiles, and the whiskers are the 0.05 and 0.95 percentiles. **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.