Fig. 4: The effective number of substitutions for each CTX-M-14 active site position. | Communications Biology

Fig. 4: The effective number of substitutions for each CTX-M-14 active site position.

From: Mapping the determinants of catalysis and substrate specificity of the antibiotic resistance enzyme CTX-M β-lactamase

Fig. 4: The effective number of substitutions for each CTX-M-14 active site position.

The k* bar plot in each panel represents the effective number of substitutions for each randomized active site position when selected for a Ampicillin resistance, b Cefotaxime resistance, and c Ceftazidime resistance, as calculated according to Eq. 2 (Methods). Each bar is color-coded based on its k* value so that residue positions that have only one functional amino acid (k* < 1.5) are red, residues that have a conservative number of potential substitutions (1.5 < k* < 4.0) are yellow, and residues that can accommodate a wide variety of substitutions (k* > 4.0) are green. The crystal structure of wild-type CTX-M-14 is shown on the right of each panel (PBD: 1YLT) and is color-coded by the same metric to display where each residue lies within the active site.

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