Fig. 3: SMdM of proteins in L. monocytogenes.
a, and d, SMdM results of membrane-bound mEos3.2-Prli42 and mEos3.2-RsbR1, respectively. Left panel: a point cloud of starting localizations clustered by the Voronoï method. Middle panel: distribution of all single-molecule displacements from the cell shown in the left panel. The red curve is the maximum likelihood estimation (MLE) outcomes using Eq. (4), yielding the DL indicated in the panel. Right (top) panel: spatial distribution of the number of displacements. Right (bottom) panel: a map of intracellular diffusivity with 50 × 50 nm2 resolution. b, c, and e, Box charts of the DL obtained from the cells of different replicates with free mEos3.2 (N = 18), mEos3.2-Prli42 (N = 8), and mEos3.2-RsbR1 (N = 40), respectively. f, The DL obtained from mEos3.2-RsbR1 in the wild-type and Δprli42 strains at pH 7.4 (N = 40 and 75, respectively) and shocked at pH 5 (N = 59 and 57, respectively) in the BHI medium (DL of the whole cell). Each dot shows the data of one cell. The box range indicates the standard deviation (SD), and the open square and dash symbols inside the boxes indicate the mean and median, respectively. (ns) is not significant and statistical significance was determined by one-way ANOVA, followed by Tukey’s posthoc test to calculate P-values. The left and right poles hold 20% of the cell’s length, and the remaining 60% is the middle region. Details of region selection are in Supplementary Fig. 6.
