Fig. 6: Association of LSH10 with and effects on ubiquitylation of the chromatin of the OSR2, WUS, ABI5, and ARL genes.

a Association of LSH10-His6 with the chromatin of the indicated target genes in the lsh10-1/LSH10-His6 plants. lsh10-1/LSH10-His6, white bars; non-specific background signal obtained in the wild-type plants that do not express the His6 epitope, gray bars. The outside probe is the qChIP probe, depicted in Fig. 5a, and located outside the EMSA probe locations. b Increase in H2B monoubiquitylation of the OSR2, WUS, ABI5, and ARL promoter chromatin in the lsh10-1 mutant plants. Wild-type plants, dark gray bars; lsh10-1, light gray bars; lsh10-2, white bars. The chromatin association of LSH10-His6 and the degree of H2B monoubiquitylation were analyzed by qChIP with probes described in Fig. 5a and listed in Supplementary Data 1. Error bars represent the SEM of n = 5 or n = 7 biological replicates with 3 technical repeats for each. The individual data points are indicated, and their numerical values are listed in Supplementary Data 6 and Supplementary Data 7. Differences between mean values assessed by the two-tailed t-test are statistically significant for the p-values *p < 0.05 and **p < 0.01; P ≥ 0.05 are not statistically significant (ns).