Table 1 Kinetic parameters and Kd values for DREB2A(244-272) variants binding to RCD1-RST(499-572).

From: Evolutionary fine-tuning of residual helix structure in disordered proteins manifests in complex structure and lifetime

Interaction

kon (µM−1 s−1)

koff (s−1)

Kd (nM)

RCD1-RST: DREB2A

240 ± 4a

0.18 ± 0.01a

0.8 ± 0.1a

RCD1-RST: DREB2A-R266G

230 ± 15

0.70 ± 0.01

3.0 ± 0.2

RCD1-RST: DREB2A-R266A

246 ± 15

0.383 ± 0.001

1.5 ± 0.1

RCD1-RST: DREB2A-D267A

222 ± 13

0.07 ± 0.01

0.32 ± 0.06

RCD1-RST: DREB2A-D267L

235 ± 19

0.030 ± 0.001

0.13 ± 0.01

  1. Recorded at 10 °C in 50 mM HEPES, 100 mM NaCl, pH 7.4. Raw data available as Supplementary Data 4.
  2. ± Standard error from fit.
  3. aFrom Staby et al.37
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