Fig. 1: The high-throughput 3D mapping for multiple samples at single-cell resolution.
From: Three-dimensional mapping in multi-samples with large-scale imaging and multiplexed post staining
a The array-fMOST pipeline, including agarose embedding, fMOST imaging and data analysis. b Demonstrating the process of multi-samples embedding. Yellow arrows indicate four-layer array arrangement of multiple samples. c The fMOST setup and components of the high-throughput serial sectioning and imaging system. d 50 μm brain slices obtained after systematic imaging. e Images of whole-brain pyramidal neuron axonal projections and cytoarchitecture from multiple samples acquired through this pipeline (30 µm thickness). f-h Magnified view of the boxed region in e is shown. i An immunohistochemical analysis of choline acetyltransferase conducted after array-fMOST whole-brain imaging of RV-labelled input neurons of mPFC. Serial-sectioned images at 50 μm intervals and enlarged images are shown on the right. Scale bars: e, 5 mm; f, 1 mm; g, 100 μm; h, 20 μm; i, 1 mm, 50 μm.
